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albumin goat  (Bethyl)


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    Structured Review

    Bethyl albumin goat
    Albumin Goat, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+albumin/Human+Albumin+Antibody/pmc13138298-4-0-3
    Average 96 stars, based on 427 article reviews
    albumin goat - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Biofabrication of synthetic human liver tissue with advanced programmable functions
    Article Snippet: Goat anti-Human Albumin , Bethyl , Cat#A80-229A; RRID: AB_67018.

    Microscopy:

    Article Title: Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9
    Article Snippet: .. Use rabbit anti-FAH (Grompe lab), goat anti-human albumin (Bethyl), goat anti-mouse albumin (Bethyl) as primary antibodies at dilution of 1/200. e. Use Alexa Fluoro 488 anti-goat IgG (Invitrogen) or Alexa Fluoro 555 anti-rabbit IgG (Invitrogen) as secondary antibodies. f. Capture the images with an Axiovert 200 microscope and a Nikon digital camera. ..

    Article Title: Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9.
    Article Snippet: .. Use rabbit anti-FAH (Grompe lab), goat anti-human albumin (Bethyl), goat anti-mouse albumin (Bethyl) as primary antibodies at dilution of 1/200. e. Use Alexa Fluoro 488 anti-goat IgG (Invitrogen) or Alexa Fluoro 555 anti-rabbit IgG (Invitro- gen) as secondary antibodies. f. Capture the images with an Axiovert 200 microscope and a Nikon digital camera. ..

    Article Title: Methods and compositions for detecting misfolded proteins
    Article Snippet: .. Ultrathin cryosections were single labeled with either rabbit anti-SERPINA1 (1:500, LabVision, Freemont, Calif.) or goat anti-human albumin (1:500, Bethyl Laboratories, Montgomery, Tex.) bridged using rabbit anti-goat antibody (1:200, Jackson Immunoresearch) and protein A coupled to 10 nm gold (Cell Microscopy Center, Utrecht, Netherlands). ..

    Staining:

    Article Title: Size- and density-dependent acoustic differential bioassembly of spatially-defined heterocellular architecture.
    Article Snippet: .. The sections were stained overnight at 4 ◦C for 1:250 primary antibody, goat anti-human albumin (Bethyl Laboratories, TX, USA), mouse anti-humanHNF-4α (Santa Cruz Biotechnology, TX, USA). ..

    Article Title: Efficient expansion and CRISPR-Cas9-mediated gene correction of patient-derived hepatocytes for treatment of inherited liver diseases.
    Article Snippet: .. Standard Histology, immunohistochemistry and immunofluorescence staining were performed as reported previously.20 Primary antibodies were as follows: rabbit anti-human GAPDH (Abcam, ab128915, 1:1000), goat anti-human-Albumin (Bethyl Laboratories, A80-229A, 1:200), goat anti-human-AAT (Bethyl Laboratories, A80-122B, 1:500), mouse anti-CK7 (Abcam, Ab9021, 1:500), rabbit anti CD31 (Abcam, ab28364, 1:200), rabbit anti-SOX9 (Millipore, Ab355, 1:1000), mouse anti-CK19 (Abcam, ab52625, 1:500), mouse anti-GFP (Santa Cruz, sc-9996, 1:200), rabbit anti-OTC (Abcam, ab203859, 1:200), mouse anti-Hnf4a (Santa Cruz, SC-374229, 1:200), rabbit anti-FAH (Cell Lab Tech, clt602-910, 1:1000), mouse anti-human CYP3A4 (Santa Cruz, SC-53850, 1:200), rabbit anti ARG1 (Genetex, gtx109242, 1:500). .. Secondary antibodies were Cy5-conjugated donkey anti-goat IgG (Jackson Lab, 1:500), FITC-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000), FITC-conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000).

    Article Title: Treatment induced clearance of hepatitis E viruses by interferon-lambda in liver-humanized mice.
    Article Snippet: .. Immunofluorescent staining of the livers was performed using rabbit anti- ORF233 and goat anti- human albumin (A80- 229A, Bethyl Laboratories, Montgomery, TX, USA) antibodies followed by Alexa Fluor- 488 or 594- conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA). .. Nuclei were counterstained with DAPI (Thermo Fisher Scientific, Waltham, MA, USA).

    Immunohistochemistry:

    Article Title: Efficient expansion and CRISPR-Cas9-mediated gene correction of patient-derived hepatocytes for treatment of inherited liver diseases.
    Article Snippet: .. Standard Histology, immunohistochemistry and immunofluorescence staining were performed as reported previously.20 Primary antibodies were as follows: rabbit anti-human GAPDH (Abcam, ab128915, 1:1000), goat anti-human-Albumin (Bethyl Laboratories, A80-229A, 1:200), goat anti-human-AAT (Bethyl Laboratories, A80-122B, 1:500), mouse anti-CK7 (Abcam, Ab9021, 1:500), rabbit anti CD31 (Abcam, ab28364, 1:200), rabbit anti-SOX9 (Millipore, Ab355, 1:1000), mouse anti-CK19 (Abcam, ab52625, 1:500), mouse anti-GFP (Santa Cruz, sc-9996, 1:200), rabbit anti-OTC (Abcam, ab203859, 1:200), mouse anti-Hnf4a (Santa Cruz, SC-374229, 1:200), rabbit anti-FAH (Cell Lab Tech, clt602-910, 1:1000), mouse anti-human CYP3A4 (Santa Cruz, SC-53850, 1:200), rabbit anti ARG1 (Genetex, gtx109242, 1:500). .. Secondary antibodies were Cy5-conjugated donkey anti-goat IgG (Jackson Lab, 1:500), FITC-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000), FITC-conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000).

    Immunofluorescence:

    Article Title: Efficient expansion and CRISPR-Cas9-mediated gene correction of patient-derived hepatocytes for treatment of inherited liver diseases.
    Article Snippet: .. Standard Histology, immunohistochemistry and immunofluorescence staining were performed as reported previously.20 Primary antibodies were as follows: rabbit anti-human GAPDH (Abcam, ab128915, 1:1000), goat anti-human-Albumin (Bethyl Laboratories, A80-229A, 1:200), goat anti-human-AAT (Bethyl Laboratories, A80-122B, 1:500), mouse anti-CK7 (Abcam, Ab9021, 1:500), rabbit anti CD31 (Abcam, ab28364, 1:200), rabbit anti-SOX9 (Millipore, Ab355, 1:1000), mouse anti-CK19 (Abcam, ab52625, 1:500), mouse anti-GFP (Santa Cruz, sc-9996, 1:200), rabbit anti-OTC (Abcam, ab203859, 1:200), mouse anti-Hnf4a (Santa Cruz, SC-374229, 1:200), rabbit anti-FAH (Cell Lab Tech, clt602-910, 1:1000), mouse anti-human CYP3A4 (Santa Cruz, SC-53850, 1:200), rabbit anti ARG1 (Genetex, gtx109242, 1:500). .. Secondary antibodies were Cy5-conjugated donkey anti-goat IgG (Jackson Lab, 1:500), FITC-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3-conjugated donkey anti-rabbit IgG (Jackson Lab, 1:500), Cy3conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000), FITC-conjugated donkey anti-mouse IgG (Jackson Lab, 1:1000).

    Labeling:

    Article Title: Methods and compositions for detecting misfolded proteins
    Article Snippet: .. Ultrathin cryosections were single labeled with either rabbit anti-SERPINA1 (1:500, LabVision, Freemont, Calif.) or goat anti-human albumin (1:500, Bethyl Laboratories, Montgomery, Tex.) bridged using rabbit anti-goat antibody (1:200, Jackson Immunoresearch) and protein A coupled to 10 nm gold (Cell Microscopy Center, Utrecht, Netherlands). ..

    Affinity Purification:

    Article Title: Differential effects of FcRn antagonists on the subcellular trafficking of FcRn and albumin
    Article Snippet: Diluted study samples and standards (0–500 ng/mL of HSA; MilliporeSigma, A3782) were added to the plates and incubated for 1 hour with 450 rpm shaking. .. The plates were washed 5 times as described above, before the detection antibody, goat anti-human albumin (polyclonal antigen affinity purified) conjugated to HRP (Bethyl, A80-129P), at a 1:32,000 dilution in 1× PBS pH 7.4, was added to all wells and the plate incubated for 1 hour with shaking. .. Last, TMB substrate (CL07, MilliporeSigma) was equilibrated to room temperature and added to all wells after washing 5 times.

    Incubation:

    Article Title: Differential effects of FcRn antagonists on the subcellular trafficking of FcRn and albumin
    Article Snippet: Diluted study samples and standards (0–500 ng/mL of HSA; MilliporeSigma, A3782) were added to the plates and incubated for 1 hour with 450 rpm shaking. .. The plates were washed 5 times as described above, before the detection antibody, goat anti-human albumin (polyclonal antigen affinity purified) conjugated to HRP (Bethyl, A80-129P), at a 1:32,000 dilution in 1× PBS pH 7.4, was added to all wells and the plate incubated for 1 hour with shaking. .. Last, TMB substrate (CL07, MilliporeSigma) was equilibrated to room temperature and added to all wells after washing 5 times.



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    a Schematic of the generation of human liver chimeric mice with hepatocyte gene KOs followed by HBV infection and FACS-based analysis. b Serial serum <t>hAlb</t> levels of FNRG mice following transplantation of mpPHHs with NT or gene-targeting sgRNAs delivered by lentiviral transduction. Each symbol is the mean; the bars represent the range across mice in each group. Mice per group: n = 6 (NT, IRF2 KO), n = 4 ( ZCCHC14 KO), n = 3 ( WDR48 KO). c Serial serum HBV DNA levels following humanization of FNRG mice are shown in ( a) of NT mice and ZCCHC14 KO mice. The dark line represents the mean for all mice per group; shading represents S.D.; each symbol represents an individual mouse and time point. Mice per group: n = 6 (NT), n = 4 ( ZCCHC14 KO). d Histograms of cell-associated HBsAg signal using FACS. Isolated mpPHHs from a representative isolation. Each histogram represents data from an individual mouse. No Ab, no primary antibody. e Percent of HBcAg-positive isolated mpPHHs measured by FACS. f HBcAg signal of HBcAg-positive isolated mpPHHs measured by FACS. g Serum HBV DNA (x-axis) is plotted against mean HBsAg signal intensity (y-axis). Each symbol represents an individual mouse; all mice from all isolations are included. For d - g , one million isolated mpPHHs from the HBV-infected, NT mouse, including in each isolation, were used for a no-primary-antibody control to establish the HBsAg and HBcAg-negative gates. Data in e and f are presented as mean ± S.D. Each symbol represents an individual mouse. Statistical significance was assessed using two-tailed unpaired t-tests with Welch's correction. * P < 0.05; ** P < 0.01.
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    a Schematic of the generation of human liver chimeric mice with hepatocyte gene KOs followed by HBV infection and FACS-based analysis. b Serial serum <t>hAlb</t> levels of FNRG mice following transplantation of mpPHHs with NT or gene-targeting sgRNAs delivered by lentiviral transduction. Each symbol is the mean; the bars represent the range across mice in each group. Mice per group: n = 6 (NT, IRF2 KO), n = 4 ( ZCCHC14 KO), n = 3 ( WDR48 KO). c Serial serum HBV DNA levels following humanization of FNRG mice are shown in ( a) of NT mice and ZCCHC14 KO mice. The dark line represents the mean for all mice per group; shading represents S.D.; each symbol represents an individual mouse and time point. Mice per group: n = 6 (NT), n = 4 ( ZCCHC14 KO). d Histograms of cell-associated HBsAg signal using FACS. Isolated mpPHHs from a representative isolation. Each histogram represents data from an individual mouse. No Ab, no primary antibody. e Percent of HBcAg-positive isolated mpPHHs measured by FACS. f HBcAg signal of HBcAg-positive isolated mpPHHs measured by FACS. g Serum HBV DNA (x-axis) is plotted against mean HBsAg signal intensity (y-axis). Each symbol represents an individual mouse; all mice from all isolations are included. For d - g , one million isolated mpPHHs from the HBV-infected, NT mouse, including in each isolation, were used for a no-primary-antibody control to establish the HBsAg and HBcAg-negative gates. Data in e and f are presented as mean ± S.D. Each symbol represents an individual mouse. Statistical significance was assessed using two-tailed unpaired t-tests with Welch's correction. * P < 0.05; ** P < 0.01.
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    a Schematic of the generation of human liver chimeric mice with hepatocyte gene KOs followed by HBV infection and FACS-based analysis. b Serial serum hAlb levels of FNRG mice following transplantation of mpPHHs with NT or gene-targeting sgRNAs delivered by lentiviral transduction. Each symbol is the mean; the bars represent the range across mice in each group. Mice per group: n = 6 (NT, IRF2 KO), n = 4 ( ZCCHC14 KO), n = 3 ( WDR48 KO). c Serial serum HBV DNA levels following humanization of FNRG mice are shown in ( a) of NT mice and ZCCHC14 KO mice. The dark line represents the mean for all mice per group; shading represents S.D.; each symbol represents an individual mouse and time point. Mice per group: n = 6 (NT), n = 4 ( ZCCHC14 KO). d Histograms of cell-associated HBsAg signal using FACS. Isolated mpPHHs from a representative isolation. Each histogram represents data from an individual mouse. No Ab, no primary antibody. e Percent of HBcAg-positive isolated mpPHHs measured by FACS. f HBcAg signal of HBcAg-positive isolated mpPHHs measured by FACS. g Serum HBV DNA (x-axis) is plotted against mean HBsAg signal intensity (y-axis). Each symbol represents an individual mouse; all mice from all isolations are included. For d - g , one million isolated mpPHHs from the HBV-infected, NT mouse, including in each isolation, were used for a no-primary-antibody control to establish the HBsAg and HBcAg-negative gates. Data in e and f are presented as mean ± S.D. Each symbol represents an individual mouse. Statistical significance was assessed using two-tailed unpaired t-tests with Welch's correction. * P < 0.05; ** P < 0.01.

    Journal: bioRxiv

    Article Title: Orthogonal CRISPR screens and human liver chimeric mice identify hepatitis B virus host factors

    doi: 10.64898/2026.07.23.740386

    Figure Lengend Snippet: a Schematic of the generation of human liver chimeric mice with hepatocyte gene KOs followed by HBV infection and FACS-based analysis. b Serial serum hAlb levels of FNRG mice following transplantation of mpPHHs with NT or gene-targeting sgRNAs delivered by lentiviral transduction. Each symbol is the mean; the bars represent the range across mice in each group. Mice per group: n = 6 (NT, IRF2 KO), n = 4 ( ZCCHC14 KO), n = 3 ( WDR48 KO). c Serial serum HBV DNA levels following humanization of FNRG mice are shown in ( a) of NT mice and ZCCHC14 KO mice. The dark line represents the mean for all mice per group; shading represents S.D.; each symbol represents an individual mouse and time point. Mice per group: n = 6 (NT), n = 4 ( ZCCHC14 KO). d Histograms of cell-associated HBsAg signal using FACS. Isolated mpPHHs from a representative isolation. Each histogram represents data from an individual mouse. No Ab, no primary antibody. e Percent of HBcAg-positive isolated mpPHHs measured by FACS. f HBcAg signal of HBcAg-positive isolated mpPHHs measured by FACS. g Serum HBV DNA (x-axis) is plotted against mean HBsAg signal intensity (y-axis). Each symbol represents an individual mouse; all mice from all isolations are included. For d - g , one million isolated mpPHHs from the HBV-infected, NT mouse, including in each isolation, were used for a no-primary-antibody control to establish the HBsAg and HBcAg-negative gates. Data in e and f are presented as mean ± S.D. Each symbol represents an individual mouse. Statistical significance was assessed using two-tailed unpaired t-tests with Welch's correction. * P < 0.05; ** P < 0.01.

    Article Snippet: ELISA plates (Thermo Fisher Scientific #4424040) were coated with goat anti-human albumin polyclonal antibody (Bethyl Laboratories #A80–129A), and bound hAlb was detected using HRP-conjugated detection antibody (Bethyl Laboratories #A80–229P).

    Techniques: Infection, Transplantation Assay, Transduction, Isolation, Control, Two Tailed Test